RN03 RNApure ultra pure total RNA rapid extraction kit
1.Product Description:
The RNAsimple Total RNA Kit adopts a new RNA extraction method based on guanidinium isothiocyanate/phenol method. The Buffer RZ specifically can remove genomic DNA and proteins from cells rapidly and efficiently, making the obtained RNA highly pure and stable.
This kit is used to separate total RNA from blood, animal cells, tissues, and plant tissues. Each spin column can process 50-100 mg tissue or 5×106 cells at a time and can process a large number of different samples simultaneously. The reaction can be completed in less than one hour, and the total RNA extracted has a higher yield, better purity, with no DNA and protein contamination, and can be used in various downstream experiments.
2.composition:
| Kit Composition | 50 times (RN0302) |
| Lysate RL (4ºC to avoid light) | 50 ml |
| Protein removal solution RE | 25ml |
| Rinsing solution RW | 10 ml(Add the specified amount of ethanol according to the directions before first use) |
| RNase-free H2O | 10 ml |
| RNase-free adsorption column RA | 50 |
| Collecting tube (2ml) | 50 |
3.Product composition, storage, concentration:
Storing the kit at room temperature for 12 months does not affect the effectiveness of use.
Storage matters:
1. Therefore, transportation and storage are carried out at room temperature (15ºC-25ºC). The lysate RL can be transported at room temperature, and can be stored at 4ºC to avoid light for a long time after receiving it. The quality of use will not be affected if stored at room temperature for 3 months.
2. Avoid volatilization, oxidation and PH changes caused by long-time exposure to the air of the reagent. Cover the solution tightly in time after use.
4.Scope of application
·RT-PCR.
·Northern Blot and Dot Blot.
·Real-Time PCR.
·polyA screening, in vitro translation, RNase protection analysis and molecular cloning.
5.Feature
·RNA purity is higher, no residual impurities, especially suitable for high purity of the downstream experiment.
·Widely used, can extract a variety of different experimental samples.
·The operation is simple, and the experiment can be completed in 1 h.
6.Experimental Procedure
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Material: 20 mg rat spleen tissue
Method: The total RNA of rat spleen tissue was isolated using the RNAsimple Total RNA Kit.
Results: Please see the above agarose gel electrophoresis picture. 2-4 μl of 100 μl eluates were loaded per lane. The electrophoresis was conducted at 6 V/cm for 30 min on a 1% agarose.
Shenzhen Smurf Technology Co., Ltd. is a high-tech enterprise certified by Shenzhen High-Tech Enterprise and Innovative Enterprise. In 2018 received the national high-tech enterprise certificate.
Many of our technologically advanced products are able to make you take less detours and do more with less money, such products are cheap and can solve the problem. To replace these head companies, takara NEB, THERMO, Vazyme, Tiangen, TransGen, Qiagen basically a hit. The price is also much more convenient.
First
All nucleic acid purification . All kinds of RNA extraction, microRNA extraction, gel recovery, plasmids, endotoxin-free plasmids, large amounts, small amounts, ultra-micro amounts, complex
LBOBIO is the first choice in this field in China, which is the closest to the world level. Simply put, we can replace 99% of the products you use with Tiangen’s better and cheaper camp. Moreover, we can do the complicated samples that basic Tiangen can’t do.
Second
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Thirdly
A variety of PCRmixe, ordinary mix, fast mix, high fidelity mix, fluorescence quantitative mix, this positioning is good quality, low price, some if not the world’s first, that is 90% of the world’s first level.
Fourth
A variety of reverse transcriptase, high temperature reverse transcriptase technology, including the most advanced one-step genome clearance, two-step genome clearance, full premixed reverse transcriptionmix branch technology, China’s leading.
The company is committed to providing life science researchers with simple, easy-to-use, trustworthy molecular biology and biochemistry reagent products and professional technical services. At the same time, in response to the disadvantages of small scale, low standard and poor product stability between batches in the bioreagent industry, the company has introduced venture capital from biopharmaceutical companies as strategic partners to introduce biopharmaceutical-grade scale production equipment, processes and procedures into the field of research reagents and provide research reagents with pharmaceutical-grade quality standards. In the future, the company will base on the frontier field of biotechnology, continuously launch new products with stable quality and reasonable price, and make continuous efforts for the development of life science in China hand in hand with the majority of researchers.
Some of the advantageous products and technology platforms are introduced as follows.
1, nucleic acid purification of various samples, especially difficult special samples such as polysaccharide polyphenol-rich plants, manure, soil and other nucleic acid separation.
2, fluorescence quantitative PCR, reverse transcription fluorescence quantitative PCR assay.
3, large-scale fermenter expression pharmaceutical grade equipment purification of scientific research tools enzymes, including a variety of DNA polymerase, T4 ligase, a variety of mutation modification reverse transcriptase, RNA enzyme inhibitors, etc.
4, PCR product cloning, TA clone ligation.
5,Western Blot Full set of related reagents and services.
6, Transfection grade, industrial grade, pharmaceutical grade large-scale plasmid production.
7, Various proteins, cytokines, enzymes and other expression purification small test, pilot test, amplification.
1. How to pick up the best among these models?
A: Tell us your request on product and almost target price, we recommend you the most suitable model. Selling you the most expensive product is not our goal, selling you the most suitable is!
2. How to place order or pay?
A: You can place order to us directly. We offer you PI with bank information and sales contract, you pay and we ship the product.
3. How to transport the product?
A: We transport the product as your requirement. Depending on the quantity and the machine you order or whether you are urgent or not, we will suggest you suitable transportation way. If you order in batch and are not very urgent, it’s best to ship by sea. If you order several items and products are not too heavy, you can choose to ship by air. For small products, courier is used commonly.
4. What kind of packing way for product?
A: Depends on which kind product you order. Most of products are packed with cartons. Some products are packed with wooden boxes. All of the packing way is based on the safety of the machine!
5. What kind of warranty will be offer?
A: For qpcr and thermal cyclers, the warranty is 24 months from the delivery date. And others are 12 months from the delivery date. During the warranty time, if any problem, we offer after-sale servies
6.Lead Times?
Lead times can vary depending on your need and freight availability. At the time of your order, a member of our team will provide you with the most accurate and current lead time.
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