PL14-DNA Extraction Kit PhasePrep EndoFree Maxi Kit
(solution-based, endotoxin-free, transfection grade)
Product Introduction:
This kit extracts plasmid DNA from cultured bacteria by alkaline lysis, using a unique solution formulation and endotoxin removal reagent.
This kit extracts plasmid DNA from culture bacteria by alkaline lysis, using a unique solution formula and endotoxin removal reagent.
The OD260/280 of the purified DNA is usually around 1.8, and the plasmid can be directly applied to cell transfection or even in vivo animal experiments.
The OD260/280 of purified DNA is usually around 1.8, and the obtained plasmids can be directly used in cell transfection and even in vivo animal experiments, which require high purity of DNA. The later stages of purification are performed in 1.5 ml.
The method is simple and does not require any special equipment, no need to pass laurel, no phenol chloroform extraction; basically, the DNA released from bacterial lysis can be completely recovered.
The plasmids released by bacterial lysis can be fully recovered without worrying about the loss of plasmid DNA. This method can extract and purify the plasmid DNA with little damage to the plasmid.
Even for large plasmids of 10kb or even 100kb or ultra-large BAC/PAC plasmids, only the alkaline lysis method can extract the plasmids. Even for large plasmids of 10kb or even 100kb or ultra-large BAC/PAC plasmids, as long as the alkaline lysis method can extract them, they can be effectively purified. The plasmid can be lysed in any small volume with concentration up to 5ug/ul. 95% superhelix ratio, no endotoxin, good transfection effect.
Product Features:
1.
The amount of plasmids extracted is related to the bacterial culture pie level, plasmid copy number and other factors. If the extracted plasmids are low copy
plasmids or large plasmids larger than 10kb, the amount of bacteriophage should be increased, and the amount of PI, P2 and PII should be increased proportionally.
The amount of PI, P2 and PII should be increased proportionally.
2.
When extracting large plasmids, you should be gentle and use tips with large openings to prevent mechanical shear damage to the DNA.
The DNA should be damaged by mechanical shearing.
3.
After centrifugation of the DNA precipitate, no obvious precipitate may be visible. If no precipitate is seen and you are concerned about DNA loss, you may retain the supernatant until the completion of the procedure.
The supernatant can be retained and identified by electrophoresis after completion of all operations to determine if the end product (hundreds of grams) is obtained.
DNA precipitates by centrifugation on the side wall of the tube and no visible clumps may be visible).
4.
The obtained plasmid DNA can be tested for concentration and purity by agarose gel electrophoresis and UV spectrophotometer.
A value of 1 corresponds to approximately 50ug/ml DNA.
This is mainly due to the different degrees of superhelical conformation of the plasmid, and is related to the length of time the extracts were incubated, and the manipulation of the extracts.
This is mainly due to different degrees of superhelical conformation plasmid swimming position, and it is related to the length of time of extract cultivation and the intensity of operation during extraction. Under the normal operation of our products, the basic superspiral DNA bands are not visible.
The rotation can exceed 95%.
5.
The exact molecular size of plasmid DNA must be linearized by enzymatic digestion and compared with DNA molecular weight Marker.
The exact molecular size of plasmid DNA can only be known after enzymatic linearization and comparison with DNA molecular weight marker. The exact size of the plasmid can not be known by electrophoresis because of the uncertainty of the swimming position of the plasmid in the loop or superhelix state.
| RNaseA(10mg/ml) | 1.3ml | -20ºC |
| Solution P1 | 130ml | 4ºC |
| Solution P2 | 100 ml | RT |
| Solution P3 | 110 ml | RT |
| Impurity scavenger A | 3 ml | RT |
| Impurity Scavenger B | 30 ml | RT |
| Botulinum toxin scavenger | 10 ml | -20ºC |
Product Page:
50 Pieces/Box
Shenzhen Smurf Technology Co., Ltd. is a high-tech enterprise certified by Shenzhen High-Tech Enterprise and Innovative Enterprise. In 2018 received the national high-tech enterprise certificate.
Many of our technologically advanced products are able to make you take less detours and do more with less money, such products are cheap and can solve the problem. To replace these head companies, takara NEB, THERMO, Vazyme, Tiangen, TransGen, Qiagen basically a hit. The price is also much more convenient.
First
All nucleic acid purification . All kinds of RNA extraction, microRNA extraction, gel recovery, plasmids, endotoxin-free plasmids, large amounts, small amounts, ultra-micro amounts, complex
LBOBIO is the first choice in this field in China, which is the closest to the world level. Simply put, we can replace 99% of the products you use with Tiangen’s better and cheaper camp. Moreover, we can do the complicated samples that basic Tiangen can’t do.
Second
TOPO cloning vector technology, do TA cloning, flat end cloning, this is not China’s first, the world’s first, the second domestic technology, all-style gold topo vector, we cite two indicators: our efficiency is 10-100 times higher than it. It takes 2 hours to complete the whole process at the fastest, we only need 15 minutes at the fastest. Less than half the price.
Thirdly
A variety of PCRmixe, ordinary mix, fast mix, high fidelity mix, fluorescence quantitative mix, this positioning is good quality, low price, some if not the world’s first, that is 90% of the world’s first level.
Fourth
A variety of reverse transcriptase, high temperature reverse transcriptase technology, including the most advanced one-step genome clearance, two-step genome clearance, full premixed reverse transcriptionmix branch technology, China’s leading.
The company is committed to providing life science researchers with simple, easy-to-use, trustworthy molecular biology and biochemistry reagent products and professional technical services. At the same time, in response to the disadvantages of small scale, low standard and poor product stability between batches in the bioreagent industry, the company has introduced venture capital from biopharmaceutical companies as strategic partners to introduce biopharmaceutical-grade scale production equipment, processes and procedures into the field of research reagents and provide research reagents with pharmaceutical-grade quality standards. In the future, the company will base on the frontier field of biotechnology, continuously launch new products with stable quality and reasonable price, and make continuous efforts for the development of life science in China hand in hand with the majority of researchers.
Some of the advantageous products and technology platforms are introduced as follows.
1, nucleic acid purification of various samples, especially difficult special samples such as polysaccharide polyphenol-rich plants, manure, soil and other nucleic acid separation.
2, fluorescence quantitative PCR, reverse transcription fluorescence quantitative PCR assay.
3, large-scale fermenter expression pharmaceutical grade equipment purification of scientific research tools enzymes, including a variety of DNA polymerase, T4 ligase, a variety of mutation modification reverse transcriptase, RNA enzyme inhibitors, etc.
4, PCR product cloning, TA clone ligation.
5,Western Blot Full set of related reagents and services.
6, Transfection grade, industrial grade, pharmaceutical grade large-scale plasmid production.
7, Various proteins, cytokines, enzymes and other expression purification small test, pilot test, amplification.
1. How to pick up the best among these models?
A: Tell us your request on product and almost target price, we recommend you the most suitable model. Selling you the most expensive product is not our goal, selling you the most suitable is!
2. How to place order or pay?
A: You can place order to us directly. We offer you PI with bank information and sales contract, you pay and we ship the product.
3. How to transport the product?
A: We transport the product as your requirement. Depending on the quantity and the machine you order or whether you are urgent or not, we will suggest you suitable transportation way. If you order in batch and are not very urgent, it’s best to ship by sea. If you order several items and products are not too heavy, you can choose to ship by air. For small products, courier is used commonly.
4. What kind of packing way for product?
A: Depends on which kind product you order. Most of products are packed with cartons. Some products are packed with wooden boxes. All of the packing way is based on the safety of the machine!
5. What kind of warranty will be offer?
A: For qpcr and thermal cyclers, the warranty is 24 months from the delivery date. And others are 12 months from the delivery date. During the warranty time, if any problem, we offer after-sale servies
6.Lead Times?
Lead times can vary depending on your need and freight availability. At the time of your order, a member of our team will provide you with the most accurate and current lead time.
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