TRIpure reagent can be an absolute replacement for Invitrogen′s TRIpure
1.Product Description:
This product is a special reagent for Real Time PCR by SYBR ® Green I chimeric fluorescence method. Heat has been activated Reagents such as HotMaster Taq DNA polymerase, dNTP, special stabilizer, optimized reaction buffer, BSA and SYBR ® Green I were premixed into a 2 Premix Type reagent suitable for Real Time PCR reaction detection, which has high sensitivity, strong specificity and good stability. When using, only by adding the template and primers and water, you can get a good standard curve in a wide quantitative area, and conduct accurate quantitative detection of the target gene, with good repetition and high credibility. Using a brand new Hotmaster Taq DNA polymerase, This enzyme differs from the general Hot-start enzyme in that, The general Hot-start enzyme only seals the enzyme activity before the temperature increases in the first step, However, Hotmaster Taq DNA polymerase closes the substrate binding site of Hotmaster Taq DNA polymerase in a thermoregulatory manner using inhibitors, At temperatures below 40ºC, Form inactive enzyme – inhibitor complexes, When the temperature rises to the primer -specific annealing temperature, Binding equilibrium moves toward the direction of template-specific primer complex formation, Thus minimizing the production of nonspecific amplification products in the whole process of PCR amplification, The accuracy of the fluorescence quantitative PCR reaction was greatly improved.
2.Specification:
| Specification | |
| Separation technology | Organic Extraction |
| Purification Time | 1 hour |
| Sample type | Bacteria, blood, cells, plant samples, tissue, virus samples, yeast |
| End product type | Total RNA, transcriptomic RNA, micro RNA |
| High throughput capability | Not compatible with high throughput applications (manual) |
| Suitable for (application) | Quantitative real-time fluorescence PCR (qPCR), reverse transcriptase PCR (RT-PCR), cDNA library construction, nuclease protection assay, Northern blotting, cloning |
| including | 100 mL TRIzol reagent |
| Number of reactions | 100 preparations |
| Product lines | TRIzol™ |
| Number | 1 |
| Specification | Graduated readable |
| Transport conditions | Room temperature |
| Raw material volume | Up to 1 g tissue, up to 1 x 10^7 cells |
| Target | Total RNA, transcriptomic RNA, miRNA |
| System type | TRIzol |
3.Product composition, storage, concentration:
TRIpure is stable at room temperature for 12 months. However, it is recommended to store at 2 to 8°C for best results.
4.Scope of application
It is suitable for rapid extraction of total RNA, DNA and protein from various animal and plant tissues/cells.
5.Feature
• Isolation of RNA, DNA and proteins from the same sample
• Excellent cracking capability and can handle even difficult sample types
• Formulations and protocols optimized for tissues, cells, serums, viruses and bacteria
Shenzhen Smurf Technology Co., Ltd. is a high-tech enterprise certified by Shenzhen High-Tech Enterprise and Innovative Enterprise. In 2018 received the national high-tech enterprise certificate.
Many of our technologically advanced products are able to make you take less detours and do more with less money, such products are cheap and can solve the problem. To replace these head companies, takara NEB, THERMO, Vazyme, Tiangen, TransGen, Qiagen basically a hit. The price is also much more convenient.
First
All nucleic acid purification . All kinds of RNA extraction, microRNA extraction, gel recovery, plasmids, endotoxin-free plasmids, large amounts, small amounts, ultra-micro amounts, complex
LBOBIO is the first choice in this field in China, which is the closest to the world level. Simply put, we can replace 99% of the products you use with Tiangen’s better and cheaper camp. Moreover, we can do the complicated samples that basic Tiangen can’t do.
Second
TOPO cloning vector technology, do TA cloning, flat end cloning, this is not China’s first, the world’s first, the second domestic technology, all-style gold topo vector, we cite two indicators: our efficiency is 10-100 times higher than it. It takes 2 hours to complete the whole process at the fastest, we only need 15 minutes at the fastest. Less than half the price.
Thirdly
A variety of PCRmixe, ordinary mix, fast mix, high fidelity mix, fluorescence quantitative mix, this positioning is good quality, low price, some if not the world’s first, that is 90% of the world’s first level.
Fourth
A variety of reverse transcriptase, high temperature reverse transcriptase technology, including the most advanced one-step genome clearance, two-step genome clearance, full premixed reverse transcriptionmix branch technology, China’s leading.
The company is committed to providing life science researchers with simple, easy-to-use, trustworthy molecular biology and biochemistry reagent products and professional technical services. At the same time, in response to the disadvantages of small scale, low standard and poor product stability between batches in the bioreagent industry, the company has introduced venture capital from biopharmaceutical companies as strategic partners to introduce biopharmaceutical-grade scale production equipment, processes and procedures into the field of research reagents and provide research reagents with pharmaceutical-grade quality standards. In the future, the company will base on the frontier field of biotechnology, continuously launch new products with stable quality and reasonable price, and make continuous efforts for the development of life science in China hand in hand with the majority of researchers.
Some of the advantageous products and technology platforms are introduced as follows.
1, nucleic acid purification of various samples, especially difficult special samples such as polysaccharide polyphenol-rich plants, manure, soil and other nucleic acid separation.
2, fluorescence quantitative PCR, reverse transcription fluorescence quantitative PCR assay.
3, large-scale fermenter expression pharmaceutical grade equipment purification of scientific research tools enzymes, including a variety of DNA polymerase, T4 ligase, a variety of mutation modification reverse transcriptase, RNA enzyme inhibitors, etc.
4, PCR product cloning, TA clone ligation.
5,Western Blot Full set of related reagents and services.
6, Transfection grade, industrial grade, pharmaceutical grade large-scale plasmid production.
7, Various proteins, cytokines, enzymes and other expression purification small test, pilot test, amplification.
1. How to pick up the best among these models?
A: Tell us your request on product and almost target price, we recommend you the most suitable model. Selling you the most expensive product is not our goal, selling you the most suitable is!
2. How to place order or pay?
A: You can place order to us directly. We offer you PI with bank information and sales contract, you pay and we ship the product.
3. How to transport the product?
A: We transport the product as your requirement. Depending on the quantity and the machine you order or whether you are urgent or not, we will suggest you suitable transportation way. If you order in batch and are not very urgent, it’s best to ship by sea. If you order several items and products are not too heavy, you can choose to ship by air. For small products, courier is used commonly.
4. What kind of packing way for product?
A: Depends on which kind product you order. Most of products are packed with cartons. Some products are packed with wooden boxes. All of the packing way is based on the safety of the machine!
5. What kind of warranty will be offer?
A: For qpcr and thermal cyclers, the warranty is 24 months from the delivery date. And others are 12 months from the delivery date. During the warranty time, if any problem, we offer after-sale servies
6.Lead Times?
Lead times can vary depending on your need and freight availability. At the time of your order, a member of our team will provide you with the most accurate and current lead time.
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